Bio-Rad iCycler MyiQ2 Two-Color Real-Time PCR Detection System

The Bio-Rad iCycler MyiQ2 is a high-performance real-time PCR (qPCR) detection platform engineered for quantitative nucleic acid analysis. Designed with two-color fluorescence detection, it allows researchers to perform multiplex assays, internal normalization, and differential gene expression studies with accuracy and reproducibility.

Built on the proven iCycler thermal cycling engine, the MyiQ2 integrates precision optics, intuitive software, and robust system design, making it suitable for molecular biology, genomics, and diagnostic research laboratories.

Technical Specifications (Typical):

• Thermal Cycler: iCycler base with advanced Peltier technology for precise temperature control

• Sample Capacity: 96-well standard PCR plate (0.2 mL wells) or compatible strip tubes

• Temperature Range: ~4°C to 99°C

• Temperature Uniformity: ±0.4°C across the block for consistent amplification

• Temperature Accuracy: ±0.25°C

• Heating/Cooling Rate: Up to 3.0°C/sec for rapid cycling

• Detection Channels: 2 independent optical channels

  • Excitation Filters: Multiple wavelength-specific excitation options

  • Emission Filters: Configured for SYBR Green, FAM, HEX, VIC, and other compatible dyes

• Dynamic Range: >9 orders of magnitude for quantification

• Data Acquisition: Continuous fluorescence measurement during each cycle for real-time quantitation

• Software:

  • Real-time monitoring of amplification curves

  • Absolute and relative quantification methods

  • Melting curve analysis for amplicon specificity

  • Baseline subtraction and automatic threshold calculation

  • Export options for Excel, text, and PDF reporting

• Computer Interface: PC-based control and analysis with USB or serial connection

Applications:

• Gene Expression Analysis: Quantify mRNA levels with high sensitivity

• SNP Genotyping: Detect single nucleotide polymorphisms with two-color assays

• Pathogen Quantification: Rapid detection and quantitation of microbial load in research samples

• Molecular Diagnostics Research: Supports assay development and validation studies

• DNA/RNA Quantitation: Analyze low-copy nucleic acid samples with precision

• Educational Use: Ideal for teaching labs needing real-time PCR capabilities

Key Advantages:

• Dual-color detection allows for multiplexing and reference dye normalization

• High sensitivity optics ensure accurate detection of low-abundance targets

• Integrated thermal cycler provides uniform well-to-well performance

• Flexible software tools for both beginner and advanced users

• Durable and proven Bio-Rad design with wide adoption in research labs

The Bio-Rad iCycler MyiQ2 Two-Color Real-Time PCR System delivers robust, reproducible performance for qPCR workflows. Its combination of powerful optical detection, precise thermal cycling, and advanced data analysis software makes it a cost-effective and reliable solution for laboratories conducting routine and advanced molecular biology research.